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. 2020 Feb 11;8:57. doi: 10.3389/fbioe.2020.00057

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Copyright © 2020 Sharma, Smits, De La Vega, Lee and Willerth.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The design and printing of a dome-shaped 3D neural tissue structure. (A) Schematic representation of Aspect Biosystems’ microfluidic printhead. (B) The Computer Aided Design (CAD) file representing dome structures. (C) Phase contrast images of day 0 printed construct showing NPCs and microspheres are dispersed throughout the fibers within the constructs (100 μm). Top-down light microscopy image of bioprinted dome shaped construct consisting of neural progenitor cells (NPCs) with bioink containing encapsulated guggulsterone microspheres. (D) Image showing the side view of a bioprinted dome and (E) showing bottom view of the construct Scale bar for (D,E) represents 10,000 μm.