Deletion of Hsp31 paralogs exhibited variable growth phenotypes. (A, B) Growth phenotype analysis. Cells grown till mid-log phase were harvested and spotted on YP rich medium containing different carbon sources, Dextrose, A and Glycerol, B. Images were captured after 38 h and 50 h of incubation in case of dextrose and glycerol, respectively. (C) Assessment of mitochondrial integrity. Yeast strains expressing MTS-mCherry grown in YP-Dextrose containing media till mid-log phase were subjected to microscopic analysis to visualize mitochondria. Scale bar (5 μm). Images were zoomed up to ~2X and represented (bottom panels).