Fig. 6.

Loss of DJ-1 paralogs promotes delayed cell-cycle progression and apoptosis.(A) Cell-cycle analysis. Yeast strains grown in liquid media were synchronized in G1 using α-factor. Released cells were collected at different time intervals and subjected to flow cytometry after PI staining. (B) Apoptotic assay. Cells grown till mid-log were stained with Annexin-V/PI followed by microscopic analysis for apoptotic induction. (C) Assessment of cell morphology and size. The yeast strains (WT, Δ31Δ34, and Δ31Δ34/Dnm1↑ cells) were microscopically examined to measure the individual cell size using Image J. The values plotted using Graph Pad Prism 5.0. (WT, n = 126; Δ31Δ34, n = 158; Δ31Δ34/Dnm1↑, n = 116). (D) Nuclear morphology analysis. The yeast cells (WT and Δ31Δ34) expressing Nup49-GFP were grown till mid-log phase and analyzed microscopically to determine nuclear morphology.