Table 2.
Percentages of the different secondary structures of SK1 and SK2 based on Gaussian curve fitting
| Structural properties | Present studya | Gao et al. (2005)a | Espinosa-Ramírez et al. (2017)a | Xiao et al. (2014)b | Georget et al. (2012)a | ||
|---|---|---|---|---|---|---|---|
| SK1 | SK2 | I | II | ||||
| Intermolecular β-sheet (%) | 23.92 ± 1.65 | 21.32 ± 1.81 | – | 36 | 42 | 24 | 40 |
| α-helix (%) | 22.10 ± 3.28 | 25.49 ± 2.93 | – | – | – | – | – |
| Random coil (%) | 17.25 ± 8.84 | 26.45 ± 4.67 | |||||
| Random coil and α-helix (%) | 39.35 ± 5.55 | 51.94 ± 7.60 | – | 35 | 33 | 49 | 39 |
| β-turns (%) | 31.21 ± 1.66 | 22.26 ± 5.37 | – | 28 | 25 | 27 | 22 |
| α-helix/intermolecular β-sheet ratio | 1.00 ± 0.04 | 1.25 ± 0.23 | 0.96 | 0.96–1.07 | 0.87 | – | – |
| amide I/amide II ratio | 1.16 ± 0.08 | 1.21 ± 0.01 | – | – | – | – | – |
I: kafirin extract from decorticated sorghum flour (dry milling) and II: kafirin extract from decorticated sorghum gluten meal (wet milling)
aEthanol extraction
bUltrasound-assisted tert-butanol extraction