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. 2020 Feb 11;11:43. doi: 10.3389/fendo.2020.00043

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Copyright © 2020 Nishimura, Kawasaki, Matsuura, Suzuki, Motojima, Baba, Ohnishi, Yamanaka, Fujitani, Yoshimura, Maruyama, Ueno, Sonoda, Nishimura, Tanaka, Sanada, Onaka, Ueta and Sakai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of Fos-immunoreactive cells in lamina I and II of lumbar segment 4 dorsal horn after intra-articular injection. Fos-immunoreactive (ir) cells (red) in the ipsilateral lamina I and II (A) and contralateral side (B) are shown (n = 5–6 per group). Scale bars = 100 μm. The ipsilateral and contralateral lamina I and II are surrounded by white dotted lines (A-a, B-a). Numbers of Fos-ir cells in the ipsilateral lamina I and II (C) and contralateral side (D) are shown. The data are presented as mean ± standard error of the mean (two-way ANOVA). **P < 0.01 compared to control. ^†P < 0.05 and ^†^†P < 0.01 compared to saline.