FIGURE 5.

Comparsion of RNA-seq and qRT-PCR results for the relative expression of predicted effector encoding genes at 0, 4, 12 hpi of S. sclerotiorum induced by C. minitans. (A) Gene expression of the 32 detected effector encoding gene of S. sclerotiorum during the early parasitized stages of S. sclerotiorum using RNA sequencing. Hollow triangles represent the five DEGs with similar gene patterns to pathogenic process; the solid black triangles indicate the eight DEGs with different patterns in parasitism process from pathogenic process. (B) Verification of 12 significantly up-regulated and 8 down-regulated effector encoding genes of S. sclerotiorum using qRT-PCR. The gene changes in transcript abundance of RNA-seq were normalized with RPKM, and for the qRT-PCR, all the data were normalized against the expression of the β-tubulin gene and the gene expression at 0 hpi was set control as 1. The related primer pairs were listed in Supplementary Table S7.