Figure 2.

Mice exposed to ⁵⁶Fe IRR at 6-month of age complete the final stage of general touchscreen training in fewer days compared to Sham mice, but perform similarly to Sham mice overall in the Pairwise Discrimination (PD) and reversal (PD rev). (a) Sham and ⁵⁶Fe IRR mice performed similarly in the first four steps of general touchscreen training with two windows: Habituation (Hab), Initiate Touch (IT), Must Touch (MT), and Must Initiate (MI). However, ⁵⁶Fe IRR mice completed the Punish Incorrect (PI) stage of general touchscreen training in fewer days than Sham mice. (b-c) Sample touchscreen images for PD and PD reversal tests. (d) Sham and ⁵⁶Fe IRR mice completed PD and PD rev in similar number of days. (e) Cumulative distribution function showing the difference in the rate of days required to complete PD between Sham and ⁵⁶Fe IRR mice. (f–h) Sham and ⁵⁶Fe IRR mice performed similarly in PD. (f) session length, (g) percent (%) correct, (h) Error number (#). (i) Cumulative distribution function showing no difference in the test days required to complete PD rev between two groups. (j–l) Sham and ⁵⁶Fe IRR mice performed similarly in PD rev. (j) Session length, (k) % correct, (l) Error #. Sham: n = 12, IRR: n = 12. Mean ± SEM. Two-way RM ANOVA in a,d,f–h,j–l, *p < 0.05, ****p < 0.0001, post hoc: Bonferroni a p < 0.05 in Sham vs. ⁵⁶Fe; Mantel-Cox test in e,i, *p < 0.05. s = seconds.