Figure 3.

Mice exposed to ⁵⁶Fe IRR at 6-month of age complete the final stage of general touchscreen testing in fewer days than Sham mice, but perform similarly to Sham in tests of rule-based learning and stimulus-response habit learning. (a) Sham and ⁵⁶Fe IRR mice performed similarly in the 4 first steps of general touchscreen training stages with three windows, including Habituation (Hab), Initiate Touch (IT), Must Touch (MT), and Must Initiate (MI). However, ⁵⁶Fe IRR mice completed the Punish Incorrect (PI) stage of general touchscreen training in fewer days than Sham mice. (b) Sample touchscreen images for Paired Associates Learning (PAL). (c–f) Sham and ⁵⁶Fe IRR mice performed similarly in PAL. (c) session length, (d) completed trials, (e) percent (%) correct, (f) Error number (#). (g,h) Sample touchscreen images for Visuomotor Conditional Learning (VMCL) train and test phases. (i) Sham and ⁵⁶Fe IRR mice performed similarly in VMCL train and test. (j) Cumulative distribution function showed no difference in days required to complete training. Distribution of Sham and ⁵⁶Fe IRR mice (n = 11/group) did not differ in days required to complete VMCL training. (k–n) Sham and ⁵⁶Fe IRR mice performed similarly in VMCL train. (k) session length, (l) completed trials, (m) % correct, (n) Error #. (o) Cumulative distribution function showed no difference in days required to complete VMCL test. Distribution of Sham and ⁵⁶Fe IRR mice (n = 11/group) did not differ in VMCLtest. (p–s) Sham and ⁵⁶Fe IRR mice performed similarly in VMCL test. (p) Session length, (q) % correct, (r) % missed, (s) Error #. Sham: n = 12 (a–f), 11 (i–s), IRR: 12 (a–f), 11 (i–s). Mean ± SEM. Two-way RM ANOVA in a,c–f,i,k–n,p–s, *p < 0.05, **p < 0.001, ****p < 0.0001, post hoc: Bonferroni a p < 0.05, a’ p < 0.01 in Sham vs. ⁵⁶Fe; Mantel-Cox test in j,o.