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. 2020 Feb 11;11:58. doi: 10.3389/fphys.2020.00058

FIGURE 3.

FIGURE 3

MRPVAT in females had a higher naïve CD4 T cell population vs. APVAT (females) and MRPVAT (males). Immunophenotyping of mesenteric resistance (MR)PVAT, thoracic aortic (A)PVAT, retroperitoneal (RP) fat, subscapular (SS) fat, and spleen, in healthy male and female SD rats. CD4 T cell subtypes (CD4+ CD25+, CD4+ OX40+, CD4+ Foxp3+, CD4+ CD45RC–, and CD4+ CD45RC+ cells) were quantified by flow cytometry. Each immune cell type is presented as absolute cell counts normalized to tissue weight (in mg). Comparisons between (A) MRPVAT and APVAT along with spleen. MRPVAT in females had a greater number of naïve CD4 T cells vs. APVAT in females and MRPVAT in males (B) MRPVAT and non-PVAT white RP fat. Females had a greater number of naïve CD4 T cells vs. males in MRPVAT (C) APVAT and non-PVAT brown SS fat. Bars represent means ± SEM for the number of animals indicated by N. A P < 0.05 by two-way ANOVA was considered statistically significant. * and # inside the stacks in the graph represent a significant difference in the specific stack of immune cells between the two fats within each sex or between the sexes within each fat, respectively.