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. 2019 Dec 11;48(3):1239–1253. doi: 10.1093/nar/gkz1157

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Mkt1 is not required for maintenance of silencing and heterochromatin at pericentromeres. (A) Schematic representation of the cen1:ade6⁺ reporter, indicating the position of the ade6⁺ insertion in centromere one relative to centromeric outer repeats (dg and dh), innermost repeats (imr) and central core (cnt). (B) Assay for silencing of the cen1:ade6⁺ reporter: silencing results in red colonies on low adenine media; loss of silencing results in pink/white colonies. (C) RT-qPCR analysis of cen(dg) transcript levels relative to act1⁺, normalized to wild-type. (D) ChIP-qPCR analysis of H3K9me2 and H3K9me3 levels at cen(dg) relative to act1⁺, normalised to wild-type. (E) Northern analysis of centromeric siRNAs (5S rRNA is a loading control).