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. 2020 Jan 16;48(3):1602. doi: 10.1093/nar/gkaa027

A transient reporter for editing enrichment (TREE) in human cells

Kylie Standage-Beier 1,2,#, Stefan J Tekel 1,#, Nicholas Brookhouser 1,3,#, Grace Schwarz 1, Toan Nguyen 1, Xiao Wang 1,, David A Brafman 1,
PMCID: PMC7026629  PMID: 31943066

Nucleic Acids Research, 2019, 47(19): e120, https://doi.org/10.1093/nar/gkz713

The reference to a recent article has been added to the Introduction:

… Some progress has been made to help enrich for edited cells, such as co-transfecting plasmids with a fluorescent reporter and using flow cytometry to isolate reporter-positive cells. Similarly, base editors fused to fluorescent proteins have been used to enrich for edited cell populations (16,58)…

58. Coelho M.A., Li S., Pane L.S., Firth M., Ciotta G., Wrigley J.D., Cuomo M.E., Maresca M., Taylor B.J.M. (2018) BE-FLARE: a fluorescent reporter of base editing activity reveals editing characteristics of APOBEC3A and APOBEC3B. BMC Biol. 16:150.

This addition does not affect the results and conclusion of the study.

May 2020: an additional correction was made to remove the sentence “However, these techniques are only reporters of transfection (RoT) and do not report on base editing activity within a cell population." from the introduction. This change has been made online but is not reflected in the print version of either the original article (https://doi.org/10.1093/nar/gkz713) or the corrigendum (https://doi.org/10.1093/nar/gkaa027).


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