Table 2.

Comparison of the APOBEC3 protein deamination preferences in the XTCX sequence context^a

Protein\preference	Nucleotides flanking the XTCX open	Nucleotides flanking the XTCX stem	‘+1’ position X for CTCX open	‘−2’ position X for XTCA open
A3A	A = T>C = G	T>C≧A = G	A≧T = G = C	C = A = G
A3B	A = C = T = G	A = T = C = G	T≧A = G = C	G = C = A
A3C	T = A = C = G	T = A = C = G	T = C = G = A	G = A = C
A3F	T≧G = C = A	A = T = G = C	A = C≧T = G	C = G≧A
A3G	C>A = T>G	C>T≧A = G	C = A = T≧G	G = C = A
A3H	A = T≧C>G	T>A = C = G	A = T>G = C	C = A = G

^aThe predicted target cytidine for deamination is indicated by an underline in each oligonucleotide. The X nucleotide in the sequence context represents a variable nucleotide. ‘>’ = greater than; ‘<’ = smaller than; ‘ = ’ = equal; ‘≧’ = greater or equal; ‘≦’ = smaller or equal. Statistical differences between groups were analyzed by using either a one-way or two-way ANOVA test, with significance having a P-value of P < 0.05.