Table 3.
Comparison of APOBEC3 protein deamination preferences with oligonucleotides containing TC targetsa
| Protein\preference | Open versus stem | Open versus bulge | Open versus loop | 10-loop versus 3-loop |
|---|---|---|---|---|
| A3A | > | > | >(ATCA); ≦ (GTCG 3-loop) | ≦ |
| A3B | = | = | = | = |
| A3C | = | = | = | = |
| A3F | ≧ | ≧ | = (ATCA); > (GTCG) | = |
| A3G | = | >(ATCA); < (GTCG) | ≧(ATCA); ≦ (GTCG) | ≧ |
| A3H | > | ≧ | ≧ | < |
aThe predicted target cytidine for deamination is indicated by an underline in each oligonucleotide. ‘>’ = greater than; ‘<’ = smaller than; ‘ = ’ = equal; ‘≧’ = greater or equal; ‘≦’ = smaller or equal. Statistical differences between groups were analyzed by using a two-way ANOVA test, with significance having a P-value of P < 0.05.