Figure 8.

Observed CR3 editing pathways in the EATRO 164 cell line grown in SDM79 (A) and SDM80 (B). U = unedited transcripts. For arrow and gRNA naming descriptors see Figure 1. Orange dots indicate edited sequence unique to the EATRO 164 cell line. Green dots indicate edited sequence unique to EATRO cells grown in SDM80 medium. Terminal dots are colored blue for reading frame 1 or magenta for reading frame 2. Boxed magenta dots have no functional start codon but are translatable into reading frame 2 with the use of an alternative start codon (UUG). + indicates that more than one mRNA form was condensed into this circle to simplify the figure (see Supplementary Figure S6). Condensed forms encode largely the same amino acid sequence with only small variants.