Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 21;48(3):1435–1450. doi: 10.1093/nar/gkz1191

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — YfiP is necessary for E. coli tRNA acp modification. (A) RP-HPLC elution profiles of tRNA nucleosides from wild type and ΔyfiP E. coli cells. acp³U elutes at 20.9 min and is missing in total tRNA from the ΔyfiP strain. (B) UV and MS spectra of purified acp³U. Left: UV spectrum of acp³U in 5 mM NH₄OAc (pH 5.3) as detected in the diode array detector coupled to LC–MS/MS. Right: MS spectrum of acp³U at 180 V fragmentor voltage. (C) Primer extension analysis of tRNA^Arg-ICG acp modification including a sequencing ladder. The reverse transcriptase arrest at C48 is missing in the ΔyfiP strain and can be restored by complementation with plasmid encoded YfiP (ΔyfiP + YfiP; vc: empty vector control).