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. 2019 Dec 21;48(3):1435–1450. doi: 10.1093/nar/gkz1191

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Determinants of acp transferase activity in E. coli YfiP. (A) Truncated or point mutated YfiP variants were expressed in E. coli ΔyfiP cells, and the degree of acp³U-47 modification of tRNA^Arg-ICG was analyzed by primer extension in comparison to the wild type (wt) and ΔyfiP cells with the empty vector (vc). (B) As shown by primer extension analysis, the YfiP homolog from Variovorax paradoxum (Vp), but not from Clostridium saccharoperbutylacetonicum (Cs), is functional as an acp³U-47 transferase in E. coli ΔyfiP.