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. 2020 Jan 20;48(3):1551–1571. doi: 10.1093/nar/gkz1186

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — MFD analysis of H3NtT:DNA interactions in presence of FRET. (A) Dy_β: FRET efficiency (E_FRET) plotted versus fluorescence weighted average donor lifetime (〈τ_D(A)〉_F). (B) FRET efficiency (E_FRET) versus fluorescence weighted average donor lifetime (〈τ_D(A)〉_F) for mutDy_β nucleosomes. (C) Total static fractions of wt Dy_β (red bar) and mutDy_β (green bar) nucleosome samples obtained by dynPDA. (D) E_α: FRET efficiency (E_FRET) plotted versus fluorescence weighted donor lifetime (〈τ_D(A)〉_F). (E) FRET efficiency (E_FRET) versus fluorescence weighted average donor lifetime (〈τ_D(A)〉_F) for mutE_α nucleosomes. (F) Total static fractions of wt E_α (red bar) and mutE_α (green bar) nucleosome samples obtained by dynPDA. The orange line represents the E_FRET–〈τ_D(A)〉_F relation for static FRET species (all parameters for the FRET lines are described in Supplementary Note 1). The magenta line represents the E_FRET–〈τ_D〉_F relation for dynamic FRET species. The comparison of the total static fractions in Dy_β and E_α nucleosome samples demonstrates the fast dynamics of the DNA arm.