Table 1.

Mean interdye distances and their relative fractions (x_i) estimated from sub-ensemble time-resolved FRET analysis for Dy_β and mutDy_β (Supplementary Note 3).

Sample	Population	[Å]	x 1*	[Å]	x 2*	x3, NoFRET
Dyβ	All FRET bursts	38.9	0.08	63.1	0.37	0.56
	D1A	41.3	0.15	63.8	0.53	0.32
	D2A	28.0	0.05	67.7	0.33	0.62
	D1D2A	38.9	0.06	63.1	0.46	0.48
mutDyβ	All FRET bursts	27.0	0.21	59.6	0.29	0.49
	D1A	33.8	0.19	57.0	0.45	0.36
	D2A	23.9	0.27	62.9	0.23	0.50
	D1D2A	35.3	0.11	60.8	0.43	0.46

*