FIGURE 6.

Time course of (A) body weight (BW, g) and (B) blood glucose concentration (BG, mg/dL) in rats treated with the NF-κB inhibitor pyrrolidine dithiocarbamate (Group DM+PDTC, n = 11) or vehicle (Group DM+V, n = 16) for 12 months. In panel (C), illustrative microphotographs (×400) of glomerulosclerosis in PAS-stained renal tissue and detection, by immunostaining, of zonula occludens-1 (ZO-1) (brown), and macrophage (ED-1) (red) in renal tissue at 12 months of DM. Quantification of (D) frequency of glomeruli with sclerotic lesions (GS,%), (E) percent area of ZO-1 and (F) macrophage density (ED-1, cells/mm²) in the glomerular area at this time. Results expressed as means ± SE. * p < 0.05 vs. DM+V.