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. Author manuscript; available in PMC: 2020 Feb 18.
Published in final edited form as: ACS Chem Biol. 2018 Sep 5;13(9):2375–2386. doi: 10.1021/acschembio.8b00520

Figure 4.

Figure 4.

Expanding the genetic code in Danio rerio embryos. (A) Diagram of UAA incorporation into proteins in zebrafish embryos through injection of the UAA, tRNAPyl, and mRNAs encoding the PylRS and Renilla luciferase (Rluc) L95TAG. (B) Structures of the UAAs incorporated into proteins in zebrafish embryos. (C) Genetic encoding of the photocaged lysine PCK allows for optical control of firefly luciferase activity in zebrafish embryos through blocking of the active site of the enzyme until UV exposure (left), as demonstrated by luminescence recovery after irradiation (right).62 Adapted with permission from J. Am. Chem. Soc. 2017, 139, 9100. Copyright 2017 American Chemical Society.