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. Author manuscript; available in PMC: 2020 Feb 18.
Published in final edited form as: ACS Chem Biol. 2018 Sep 5;13(9):2375–2386. doi: 10.1021/acschembio.8b00520

Figure 6.

Figure 6.

Proteome labeling using Drosophila and mice with an expanded genetic code. (A) Diagram of proteome labeling in fruit flies using cyclopropene-lysine (CPK) incorporation into fly proteins.85 (B) Structure of CPK and PropK. (C) Stochastic incorporation of CPK into proteins was controlled by expression of the PylRS/tRNAPyl under control of nos-vp16, limiting expression of the PylRS/tRNAPyl to germline cells in the ovary. When labeled, fluorescence was restricted to germline cells with no incorporation of CPK in the somatic follicular epithelium.83 (D) Diagram of proteome labeling in the mice brain using PropK. (E) Cell-type specific incorporation of PropK and labeling with an azido-fluorophore. α-GFAP is a glial cell specific protein and α-NeuN is a neuron specific nuclear protein. Adapted with permission from Nat. Biotechnol. 2014, 32, 445, Nat. Biotechnol. 2014, 32, 465, and Nat. Biotechnol. 2017, 36, 156. Copyright 2014 and 2017 Nature Publishing Group.