Table 1.
List of the photochemical properties of some of the new caging groups mentioned in the development section. λmax is the absorption maximum; ϵ is the molar extinction coefficient at the absorption maximum; Solvents used during photolysis: PBS (phosphate buffered saline pH=7.4), Tris is trisaminomethane buffer pH=7.5; φu is the quantum yield of decaging; δu = ϵ × φu is the decaging efficiency for single photon irradiation and is the product of the quantum yield and extinction coefficient; δu2P = δa × φu is two photon decaging action cross section for two photon irradiation where δa is the two photon absorption cross section (1 GM = 10−50 cm4 s photon−1).
| caged compound | λmax/nm | ϵ/mM−1cm−1 | solvent | φu/10−2 (λ/nm) | δu/M−1cm−1 | δu2P/GM (λ/nm) |
|---|---|---|---|---|---|---|
| 1a | 472 | 31 | 1:1 CH3CN:Tris | 0.12 (365) | 320 | - |
| 1c | 487 | 33 | 1:1 CH3CN:Tris | 0.07 (365) | 2 | - |
| 2 | 492 | 30 | 1:1 CH3CN:Tris | 0.24 (505) | 58 | - |
| 4b | 503 | 32 | MeOH | <0.001 (505) | 0.07 | - |
| 5a | 450 | 43 | PBS | 39 (450) | 16800 | 0.5 (900) |
| 6 | 407 | 29 | DMSO | 16 (405) | 4558 | 6 (760) |
| 7a | 430 | 30 | 9:1 MeOH:H2O | 45 (450) | 13500 | 26 (730) |
| 7b | 490 | 30 | 9:1 MeOH:H2O | 40 (450) | 12000 | - |
| 9 | 544 | 45 | 19:1 PBS:CH3CN | 0.01 (540) | 7 | - |
| 10a | 538 | 61 | MeOH | 28 (507) | 17056 | - |
| 11 | 661 | 65 | MeOH | 41 (532) | 3 | - |
| 12a | 668 | 40 | DMSO | - | - | - |
| 15a | 397 | 8 | PBS | 15 (405) | 1125 | 11 (800) |
| 16 | 400 | 11 | 19:1 PBS:DMSO | 22 (412) | 2500 | 20 (800) |
| 17a | 443 | 30 | MeOH | 1 (355) | 60 | |
| 19 | 440 | 66 | PBS | 23 (410) | - | 350 (810) |
| 20 | 362 | 19 | C6D6 | 30 (355) | 5631 | 120 (680) |