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. 2020 Jan 29;204(5):1322–1333. doi: 10.4049/jimmunol.1900053

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Copyright © 2020 by The American Association of Immunologists, Inc.

This article is distributed under The American Association of Immunologists, Inc., Reuse Terms and Conditions for Author Choice articles.

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FIGURE 3. — The effect of Roquin-1 deficiency on H/R-induced mø polarization. (A) Flow cytometry analysis of mø polarization (n = 6). (B and C) ELISA analysis of the cytokines of M1/M2 in supernatants (n = 6). (D) Venn diagram displaying Roquin-1 computationally predicted to target AMPKα by four different prediction algorithms: String, BioGRID, GeneMANINA, and Hitpredict. (E) WB analysis of the phosphorylation of AMPKα, mTOR, and STAT3 after H/R supernatants stimulation and/or Roquin-1 interference (n = 6). The data are expressed as the mean ± SD. *p < 0.01, **p < 0.01, ***p < 0.001, ****p < 0.0001.