Table II. PCR system and condition.

PCR System
Ingredient	Usage (μl)
DEPC water	Up to 50
10× buffer	5
2 mM dNTPs	4
Primer	2
Template plasmid	0.5
KOD plus	1

Reaction Condition
Step	Condition	No. of Cycles
Denaturation	95°C, 1 min	1
Extension	95°C, 40 s	35
	60°C, 1 min
	68°C, 2 min
Extension	68°C, 10 min	1