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Exercised animals display a decrease in Mbnl2 protein levels. Total protein was isolated from gastrocnemius muscle at the conclusion of the 10-week study. Protein levels of Mbnl1 and Mbnl2 were detected by Western blot and normalized to total protein as determined by Coomassie (representative Coomassie shown). Statistical analysis was conducted using an unpaired t-test. Error bars indicate ± SD. *P < .05
