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. 2020 Jan 31;10:1720. doi: 10.3389/fpls.2019.01720

Figure 1.

Figure 1

Schematic diagram of CRISPR/Cas9 plant transformation vector pKSE401. Oligonucleotides of gRNA sequence were annealed and then inserted at the BsaI-digestion site between the pU6-26 promoter and gRNA scaffold.