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. 2020 Feb 17;18:31. doi: 10.1186/s12951-020-00589-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Uptake of differently charged LNCs by the cells in GBM microenvironment. a Representative example of flow cytometry analysis on cell suspension from GBM tissue incubated overnight with 100 nm negative, neutral and positive DiD-LNC formulations at 50 ng/ml DiD concentration. The gating strategy is reported in the upper panels. DiD⁺ cells (blue histograms) were assessed among BMDM, MG, CD45⁻ cells and compared to the signal of Blank-LNCs (red histogram) among the same populations. b The histograms show mean ± SE of 3 independent experiments, performed as described in (A). The percentage of DiD⁺ cells was calculated in BMDM (green), MG (blue), CD45⁻ cells (pink), PMN (yellow), and lymphocytes (grey), setting the gate on Blank-LNC control. PMNs were gated as CD14⁻ SSC^high cells and lymphocytes as CD14⁻ SSC^low cells. Student t-test was performed, *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001