Fig. 1.

Study design: 300 samples were extracted from 25 donors. 2 sample replicas were processed from each donor by both technicians with three different methodologies. Quantification was performed for all samples by spectrofotometry (Infinity Tecan F200). A selection of the samples were also quantified by Ribogreen technique (n = 42). Expression analysis was performed for selected samples for 3 mRNAs and 3 miRNAs