Fig. 10.

ONT sequencing ROC curves. Curves are shown for each of the 14 assays tested (faceted by column), split over the 5 steps of the dilution series (faceted by rows). Replicates are kept separated for this analysis. The read count cutoff for calling positive/negative is log10 scaled. The area under the curve (AUC) is calculated using the ‘step’ method. Most assays achieve a 100% sensitivity and 0% FPR near a read count cutoff of 10 for the highest 3 concentrations (3, 4, and 5), and many achieve this AUC at the lowest 2 (1, 2), with the exception of PRC_30