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. 2020 Feb 17;21:166. doi: 10.1186/s12864-020-6557-5

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 12 — ONT sequencing PR and ROC curves for assays having a real time PCR FN or FP. Metrics for these particular assays are not significantly changed when separating out organisms per assay and in most cases, analysis of sequenced amplicons produced by these assays results in perfect precision, recall, and FDR. Refer to Tables 4 and 11 for additional interpretation