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. 2020 Feb 17;21:166. doi: 10.1186/s12864-020-6557-5

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Heat map of sequence read counts from 14 plex real time PCR reactions from samples spiked with all strains (48 h data). Heat map of read counts (log10 scale) of Set 2, mixed agent multiplex amplicon sequencing on ONT platform. a) Results analyzed after the first 10 min of ONT data collected and b) Results analyzed after the full 48 h runtime. Organism and corresponding strains are indicated across the top, condition and spiking concentrations (CFUs) (10 fold dilution steps 5 through 1) are along the right side (numbers in Table 2), and replicate number along the left side. Conditions are as follows: CB cocktail buffer, CF clean filter, and DF dirty filter. The x-axis indicates assay (or amplicon reference)