Table 1.
Multiplex strategy for 513 total spiked samples
| Set | Organism(s) | Strain | Dilution Steps | Matrices | Replicates | Total Barcodes | Flowcell# |
|---|---|---|---|---|---|---|---|
| 1 | Francisella tularensis | 239 | 5 | 3 | 3 | 45 | 1 |
| Francisella tularensis | 240 | 5 | 3 | 3 | 45 | 2 | |
| Francisella tularensis | 241 | 5 | 3 | 3 | 45 | 3 | |
| Yersinia pestis | 113 | 5 | 3 | 3 | 45 | 4 | |
| Yersinia pestis | 114 | 5 | 3 | 3 | 45 | 5 | |
| Burkholderia mallei | 164 | 5 | 3 | 3 | 45 | 6 | |
| Burkholderia pseudomallei | 197 | 5 | 3 | 3 | 45 | 7 | |
| Bacillus anthracis | 708-gi | 5 | 3 | 3 | 45 | 8 | |
| Bacillus anthracis | 708-live | 5 | 3 | 3 | 45 | 9 | |
| 2 | All agents (known, amplified) | all 9 | 4 | 3 | 3 | 36 | 10 |
| All agents (blinded, amplified) | all 9 | 4 | 3 | 3 | 36 | 11 | |
| All agents (known, unamplified) | all 9 | 4 | 3 | 3 | 36 | 12 |
Set 1 is composed of 405 samples, split into 9 single agent cocktails with 8 unique agents among them. Set 2 is composed of 108 samples, split into 3 combined agent cocktails containing all 8 unique agents (708-gi, not 708-live). In this study, cocktails refers to samples suspended in buffer solution