Fig. 4.

qRT-PCR and sequence analysis of heterozygous individuals. a Relative mRNA expression of EDNRB2 in individuals of three different genotypes. b Chromas files of heterozygotes at the 14-bp insertion site in the DNA sequence. The blue and red frames represent the DNA sequences in heterozygote individuals corresponding to the wt and insertion alleles, respectively. c The cDNA sequence of the insertion position of homozygous and heterozygous gray geese. The cDNA sequence of heterozygous individuals was the same as that of homozygous gray geese. No insertion was observed. The black arrow indicates the boundary between exon 3 and exon 4. The red arrow represents the location of the 14 bp insertion