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. 2020 Jan 7;19(3):1641–1648. doi: 10.3892/etm.2020.8426

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Copyright: © Luo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effects of miR-133a on glioma cell proliferation and metastasis. (A) Relative expression levels of miR-133a and XIST in glioma cells following transfection with miR-133a mimics or inhibitor. (B) Cell viability following transfection with miR-133a mimics or inhibitor assessed by MTT assay. (C) Migration (magnification, ×16) and (D) invasion (magnification, ×100) of cell lines detected by scratch-wound healing and Transwell migration assays, respectively. (E) Western blotting showing the expression of E-cadherin, N-cadherin, α-catenin and vimentin in glioma cells following transfection with miR-133a inhibitor or mimics. (F) Expression level of SOX4 transcription factor. n=3. *P<0.05, **P<0.01 and ***P<0.001. miR, microRNA; XIST, X-inactive specific transcript; SOX, SRY-box; NC, negative control; OD, optical density.