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Binding affinity between SOX4, XIST and miR-133a. (A) Predicted complementary binding sites between SOX4 or XIST and miR-133a. (B) Binding affinity was detected via dual luciferase reporter assays using wild-type or mutant forms of XIST and miR-133a. (C) Dual luciferase activity demonstrating binding between miR-133a and SOX4 with wt or mut forms. n=3. ***P<0.001. SOX, SRY-box; XIST, X-inactive specific transcript; miR, microRNA; wt, wild-type; mut, mutant; NC, negative control.
