Fig. 3.

miR-181b inhibits LPS-induced tissue factor activity in THP-1. THP-1 cells were transfected with miR-181b and stimulated with LPS for 2 h to assess asTF and flTF mRNA (a, b) or 6 h for TF activity (c). THP-1 were transfected with a control mimic or miR-181b and then stimulated with LPS for 1 h. Cytoplasmatic extracts (d, upper panel) and nuclear extracts (d, lower panel) were then subjected to western blot analysis using antibodies against phospho-NFκB (phospho p65), NFκB (p65), histone H3 (H3), or GAPDH (bands show samples with equal loading of the same gel. The original blots can be found in Additional file 3: Figure S3). KPNA4 mRNA (e), PTEN mRNA (f) and protein (g) in THP-1 transfected with a control miR or miR-181b. THP-1 were transfected with a control siRNA or siRNA against PTEN. PTEN knock down was confirmed via quantification of PTEN mRNA (h) and led to reduced mRNA levels of flTF (i) and asTF (j). n ≥ 3, groups were compared by 2-way ANOVA test with global p-values for treatment with LPS and Sidak’s multiple comparison post hoc test (a, b) or student’s t-test