Fig. 2.

Uptake of ECVs by NPCs. HBMEC were treated with HIV and/or Aβ and ECVs were isolated as in Fig. 1; however, Aβ (1–40) HiLyte was used instead of non-florescent Aβ (1–40). Then, human NPCs were exposed to HBMEC-derived ECVs for 24 h, with selected cultures pretreated with 500 nM FPS-ZM1 (FPS) for 2 h followed by cotreatment with the isolated ECVs. Images were performed by confocal microscopy z-stacking. a Transferred Aβ (1–40) HiLyte (green) from brain endothelial ECVs to NPCs. Representative images from three experiments. DRAQ5 staining (red) visualizes the NPC nuclei. Scale bar: 10 μm. b Quantification of total Aβ (1–40) HiLyte fluorescence in recipient NPCs from the confocal images. Values are mean ± SEM; n = 8–11. c Quantification of nuclear Aβ (1–40) HiLyte fluorescence in recipient NPCs from the confocal images. Values are mean ± SEM; n = 47–86 from randomly selected images. Statistically significant at *p < 0.05, **p < 0.01, or ****p < 0.0001