Fig. 3.

Introducing the conresponding genes back into the mutants surpress their SDS-sensitive and high intracellular ROS levels. a Complementation of the constructed expression plasmid in the sensitivity of the conresponding mutant to 0.03% SDS. b Intracellular ROS levels of the 11 indicated SDS-sensitive gene mutants in response to SDS stress. Strains containing the indicated plasmid were cultured in SD-URA over night for the complementary assay. To analyze the intracellular ROS levels, strains containing the indicated plasmid were first cultured to log-phase before being shifted to YPD with 0.015% SDS for additional 2 hours before they were collected for measurement of intracellular ROS levels stained by the dihydroethidium. The value is the average of three independent assays for each strain