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. 2020 Feb 17;20:52. doi: 10.1186/s12935-020-1141-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — KMT9 controls expression of genes involved in the organization of organelles, cells death and cell proliferation. a Venn diagram showing overlap and number of genes/proteins in A549 cells that are differentially expressed upon RNAi mediated knock-down of KMT9α (log2(fold-change) > ± 0.26). In total, 460 targets are concomitantly up- or down-regulated on RNA and protein level upon knock-down of KMT9α. Enriched GO_cellular components b and GO_biological processes c gene sets obtained for the indicated 460 KMT9α-regulated target genes. d Heat map displaying mRNA levels of the 460 KMT9α-regulated genes involved in cell proliferation (GO:0042127) in A549 cells treated with siControl or siKMT9α#1. e RNA sequencing reads (left panel) and mass spectrometry volcano plot (right panel) for the indicated genes and proteins are represented exemplarily. f Western blot displaying expression of the target proteins indicated in e upon knock-down of KMT9α in A549 cells. The indicated antibodies were used. g Quantitative real-time PCR analysis of the mRNA expression of the target genes displayed in e after knock-down of KMT9α. Data represent means + standard deviation. Significance was accessed by two-tailed t test, n = 3 (TIMP2 n = 6)