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. 2019 Dec 31;19(3):1633–1640. doi: 10.3892/etm.2019.8398

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Involvement of p-PI3K/p-Akt and p-mTOR on the effects of cisplatin and ghrelin. (A) Phosphorylation of PI3K/Akt/mTOR was detected by western blotting. (B and C) Expression of Bax and Bcl-2 was detected by western blotting, PI-3K inhibitor, LY294002 (10 µM) or rapamycin (20 nM) was added 30 min before ghrelin and/or cisplatin treatment. (D) Apoptosis of MDA-MB-231 cells was analysed by flow cytometry. *P<0.05 vs. control; ^#P<0.05 vs. cisplatin treated alone; **P<0.05 vs. cisplatin + Ghrelin. Bax; B-cell lymphoma 2-associated X; Bcl2, B-cell lymphoma 2; Cisp, cisplatin; Con, control; FITC-A, Annexin V-Fluorescein isothiocyanate; Ghr, ghrelin; PI3K, phosphoinositide 3-kinase; mTOR, mammalian target of rapamycin; siRNA, small interfering RNA.