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. 2020 Feb 18;48:8. doi: 10.1186/s41182-020-00197-9

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© The Author(s) 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Specificity of real-time PCR for primers used to detect of E. faecalis, E. faecium, and VRE stains. Melting curve analysis showing the melting temperature peaks (Tm) of E. faecalis (a), E. faecium (b), and VRE stains (c). a blacks’ lane, E. faecalis NCTC13779; red lane, Streptococcus pneumoniae ATCC49619. b blacks’ lane, E. faecium NCTC7174; red lane, Streptococcus pneumoniae ATCC49619. c blacks’ lane, VRE strains; red lane, Streptococcus pneumoniae ATCC49619