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. 2020 Jan 3;19(3):1687–1694. doi: 10.3892/etm.2020.8422

Figure 3.

Figure 3.

Treatment with TSA reverses EMT in MCF-7 cells. (A) MCF-7 cells were treated with or without TSA, and the mRNA level of E-cadherin, vimentin and SLUG were analyzed by reverse transcription-quantitative polymerase chain reaction. **P<0.01 vs. control. (B) MCF-7 cells were incubated with or without TSA, and subsequently the protein levels of E-cadherin, vimentin and SLUG were analyzed by western blot analysis. (C) MCF-7 cells were treated with or without TSA, and the cellular location of E-cadherin (green) was examined by confocal microscopy. Scale bar, 20 µm. TSA, trichostatin A; EMT, epithelial-mesenchymal transition; E-cadherin, epithelial cadherin; SLUG, zinc finger protein SNAI2.