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. 2020 Jan 3;19(3):1687–1694. doi: 10.3892/etm.2020.8422

Figure 4.

Figure 4.

TSA-mediated suppression of SLUG is involved in reversing EMT. (A) pcDNA-3.1 and pcDNA-SLUG were expressed in MCF-7 cells, and cells with pcDNA-SLUG were treated with or without TSA. Subsequently, the mRNA levels of SLUG, E-cadherin and vimentin were examined by reverse transcription-quantitative polymerase chain reaction. ##P<0.01 and ###P<0.001 vs. pcDNA-3.1. **P<0.01 and ***P<0.001 vs. pcDNA-SLUG. (B) pcDNA-3.1 and pcDNA-SLUG were expressed in MCF-7 cells, and cells with pcDNA-SLUG were incubated with or without TSA. Subsequently, the protein levels of SLUG, E-cadherin and vimentin were examined by western blot analysis. (C) SLUG siRNA or control siRNA was transfected in MCF-7 cells, and images were captured of the invasive and migrated cells. Scale bar=200 µm. *P<0.05 and ***P<0.001 vs. control. (D) SLUG siRNA or control siRNA were transfected in MCF-7 cells, and subsequently the expression levels of SLUG, E-cadherin, and vimentin protein were examined by western blot analysis. TSA, trichostatin A; EMT, epithelial-mesenchymal transition; SLUG, zinc finger protein SNAI2; E-cadherin, epithelial cadherin; siRNA, small interfering RNA.