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. 2020 Jan 3;19(3):1655–1664. doi: 10.3892/etm.2020.8421

Figure 2.

Figure 2.

Effects of miR-21 inhibitor on cell viability and LDH release in H9c2 cells subjected to H/R injury in the presence or absence of Sal. H9c2 cells were transfected with either an miR-21 inhibitor or inhibitor-NC. Following transfection, cells were pretreated with Sal, and were subsequently maintained under hypoxia for 6 h, followed by 12 h of reoxygenation. (A) Cell viability was detected using an MTT assay. (B) An LDH release assay was conducted using a commercial kit. (C) miR-21 expression was analyzed using reverse transcription-quantitative PCR. U6 was used as an internal control. Data are presented as the mean ± standard deviation of three experiments. **P<0.01 vs. the control group; #P<0.05, ##P<0.01 vs. the H/R group; &P<0.05 and &&P<0.01 vs. the Sal + H/R control group. H/R, hypoxia/reoxygenation; miR-21, microRNA-21; Sal, salidroside; NC, negative control; LDH, lactate dehydrogenase.