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. 2019 Dec 20;19(3):1878–1886. doi: 10.3892/etm.2019.8359

Figure 6.

Figure 6.

Treatment with the autophagy inhibitor, 3-MA, eliminates the protective properties of RV in malignancy. B16-F10 cells were treated with 100 µM RV with or without cotreatment with 10 mM 3-MA for 24 h. (A) Cell Counting Kit-8 assay was used to assess viability. (B) Percentage of apoptotic B16-F10 cells following the various treatments. (C) Annexin V-FITC/PI flow cytometry analysis of B16-F10 cells following RV, RV + 3-MA, or Con treatment. (D) Representative micrographs and (E) quantification of migratory B16-F10 cells in the lower chamber of the Transwell plates following treatment (magnification, ×20). Data are presented as the mean ± SEM. **P<0.01 vs. Con; #P<0.05 vs. RV. 3-MA, 3-methyladenine; PI, propidium iodide; RV, resveratrol.