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. 2020 Jan 8;19(3):1779–1788. doi: 10.3892/etm.2020.8437

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Validation of six hub genes through experiments. (A) Validation of hub genes by reverse transcription-quantitative PCR. Boxplots indicate the medians and dispersions of 47 HCC and their adjacent tissue samples. P-values were determined using a Student' t-test. ***P<0.001 and ****P<0.0001 with comparisons shown by lines. (B) Western blotting detection of hub genes. Lysates from 3 pairs of HCC and adjacent tissues were subjected to western blotting with antibodies against CCNB2, CDC20, MAD2L1, MCM2, CENPF and BUB1B. β-actin was used as the reference gene. HCC, hepatocellular carcinoma; CCNB2, cyclin B2; CDC20, cell division cycle 20; MAD2L1, mitotic arrest deficient 2 like 1; MCM2 minichromosome maintenance complex component 2; CENPF, centromere protein F; BUB1B, BUB mitotic checkpoint serine/threonine kinase B.