Table II.
Evaluation of the expression of collagen types I and III in primary human gingival fibroblasts after 24 h of incubation with gingival retraction agents diluted with the cell culture medium (DMEM) to 1:20 concentration.
| Collagen type I | Collagen type III | |||
|---|---|---|---|---|
| Gingival retraction agent | Positive cells, % | Reaction intensity | Positive cells, % | Reaction intensity |
| Control | 98 | ++/+++ | 97 | +++ |
| Afrin | 100 | ++ | 100 | + |
| Visine | 100 | + | 100 | + |
| Starazolin | 100 | + | 100 | −/+ |
| Neosynephrin | 100 | ++ | 100 | + |
| Adrenaline 0.05% | 100 | + | 100 | + |
| EG-1 | 100 | ++/+++ | 100 | +++ |
| EG-2 | 100 | ++ | 100 | ++/+++ |
| EG-3 | 100 | ++ | 100 | ++/+++ |
The intensity of immunocytochemical staining was rated as follows: i) (−) Negative (no stained reaction), ii) (+) weak; iii) (++) moderate; iv) (++/+++) higher than moderate; and v) (+++) strong. EG, experimental gel.