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. 2020 Feb 17;53:9. doi: 10.1186/s40659-020-00275-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — miR-34-5p was a target miRNA of SNHG7. a The predicted binding sites of miR-34a-5p to SNHG7 sequence using starBase 2.0. b The luciferase activity of 293T cells co-transfected with miR-34a-5p and luciferase reporters containing SNHG7-wt or SNHG7-mut was detected using luciferase reporter assay. c The luciferase activity of 293T cells co-transfected with anti-miR-34a-5p and luciferase reporters containing SNHG7-wt or SNHG7-mut was detected using luciferase reporter assay. d The expression of SNHG7 was detected in the samples bound to the Ago2 antibody by RIP assay. e The expression of SNHG7 was detected using qRT-PCR in samples pulled down by biotinylated miR-34a-5p or negative control (NC). f The expression of miR-34a-5p was detected in OA cells (IL-1β) transfected with control, vector, SNHG7, scramble and siSNHG7 by qRT-PCR. g Pearson’s correlation analysis revealed the significant negative correlation between SNHG7 and miR-34a-5p expression. *P < 0.05