Skip to main content
. 2020 Jan 14;19(3):2059–2066. doi: 10.3892/etm.2020.8449

Figure 3.

Figure 3.

GLI1 is a direct target gene of miR-584. (A) The 3′-UTR of GLI1 mRNA includes a highly conserved binding site for miR-584. (B) 293T cells were co-transfected with miR-584 mimics or inhibitors and wt GLI1 mRNA 3′-UTR or mut GLI1 mRNA 3′-UTR. Luciferase activity was analyzed following 24 h of transfection. (C) mRNA expression of GLI1 was analyzed in HeLa and CaSki cells. β-actin was used as an internal control. (D) The protein expression levels of GLI1 were detected in HeLa and CaSki cells. β-actin served as an internal control. (E) Relative GLI1 mRNA expression was analyzed in tumor tissues the corresponding adjacent normal tissues by reverse transcription-quantitative PCR. β-actin served as internal control. (F) The correlation between the mRNA expression of GLI1 and miR-584 in cervical cancer tissue samples analyzed by Pearson's correlation analysis. *P<0.05. 3′UTR, 3′-untranslated region; wt, wild-type; mut, mutant; miR, microRNA; miR-NC, mimic negative control; anti-NC, inhibitor negative control; GLI1, glioma-associated oncogene 1.