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. 2020 Jan 14;19(3):2059–2066. doi: 10.3892/etm.2020.8449

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — GLI1 is a functional target gene of miR-584. (A) HeLa cells were co-transfected with miR-584 mimics and GLI1 plasmid or empty plasmid. The protein expression levels of GLI1 were then analyzed by western blotting. (B) Cell proliferation of HeLa cells was analyzed using a Cell Counting Kit-8 assay. (C) Cell proliferation of HeLa cells was analyzed using a colony formation assay. (D) A Transwell assay was used to analyze the migration and invasion capability of HeLa cells (Scale bar, 100 µm; magnification, ×100). *P<0.05. miR, microRNA; GLI1, glioma-associated oncogene 1.